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Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2016 Sep 09; Vol. 478 (1), pp. 343-348. Date of Electronic Publication: 2016 Jul 11. - Publication Year :
- 2016
-
Abstract
- Transglutaminase (TGase) catalyzes protein cross-linking reactions essential for several biological processes. In differentiating keratinocytes, TG1 (keratinocyte-type) is crucial for the cross-linking of substrate proteins required for the complete formation of the cornified envelop, a proteinaceous supermolecule located in the outermost layer of the epidermis. TG1 expressions and its substrate were induced in cultured keratinocytes at differentiation-stage specific manner. In the cultured keratinocytes, we used the TG1-specific substrate peptide, which enables the specific detection of enzymatic activity to investigate its induction patterns. As a further application of the substrate peptide, several substrate candidates of TG1 that may be essential for cornified envelope formation were identified and characterized.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 478
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 27416753
- Full Text :
- https://doi.org/10.1016/j.bbrc.2016.07.051