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Urothelial ATP exocytosis: regulation of bladder compliance in the urine storage phase.
- Source :
-
Scientific reports [Sci Rep] 2016 Jul 14; Vol. 6, pp. 29761. Date of Electronic Publication: 2016 Jul 14. - Publication Year :
- 2016
-
Abstract
- The bladder urothelium is more than just a barrier. When the bladder is distended, the urothelium functions as a sensor to initiate the voiding reflex, during which it releases ATP via multiple mechanisms. However, the mechanisms underlying this ATP release in response to the various stretch stimuli caused by bladder filling remain largely unknown. Therefore, the aim of this study was to elucidate these mechanisms. By comparing vesicular nucleotide transporter (VNUT)-deficient and wild-type male mice, we showed that ATP has a crucial role in urine storage through exocytosis via a VNUT-dependent mechanism. VNUT was abundantly expressed in the bladder urothelium, and when the urothelium was weakly stimulated (i.e. in the early filling stages), it released ATP by exocytosis. VNUT-deficient mice showed reduced bladder compliance from the early storage phase and displayed frequent urination in inappropriate places without a change in voiding function. We conclude that urothelial, VNUT-dependent ATP exocytosis is involved in urine storage mechanisms that promote the relaxation of the bladder during the early stages of filling.
- Subjects :
- Animals
Cells, Cultured
Humans
Male
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Electron, Transmission
Nucleotide Transport Proteins genetics
Nucleotide Transport Proteins metabolism
Urinary Bladder cytology
Urinary Bladder ultrastructure
Urinary Tract metabolism
Urination
Urothelium cytology
Urothelium ultrastructure
Adenosine Triphosphate metabolism
Exocytosis
Urinary Bladder metabolism
Urothelium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27412485
- Full Text :
- https://doi.org/10.1038/srep29761