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Urothelial ATP exocytosis: regulation of bladder compliance in the urine storage phase.

Authors :
Nakagomi H
Yoshiyama M
Mochizuki T
Miyamoto T
Komatsu R
Imura Y
Morizawa Y
Hiasa M
Miyaji T
Kira S
Araki I
Fujishita K
Shibata K
Shigetomi E
Shinozaki Y
Ichikawa R
Uneyama H
Iwatsuki K
Nomura M
de Groat WC
Moriyama Y
Takeda M
Koizumi S
Source :
Scientific reports [Sci Rep] 2016 Jul 14; Vol. 6, pp. 29761. Date of Electronic Publication: 2016 Jul 14.
Publication Year :
2016

Abstract

The bladder urothelium is more than just a barrier. When the bladder is distended, the urothelium functions as a sensor to initiate the voiding reflex, during which it releases ATP via multiple mechanisms. However, the mechanisms underlying this ATP release in response to the various stretch stimuli caused by bladder filling remain largely unknown. Therefore, the aim of this study was to elucidate these mechanisms. By comparing vesicular nucleotide transporter (VNUT)-deficient and wild-type male mice, we showed that ATP has a crucial role in urine storage through exocytosis via a VNUT-dependent mechanism. VNUT was abundantly expressed in the bladder urothelium, and when the urothelium was weakly stimulated (i.e. in the early filling stages), it released ATP by exocytosis. VNUT-deficient mice showed reduced bladder compliance from the early storage phase and displayed frequent urination in inappropriate places without a change in voiding function. We conclude that urothelial, VNUT-dependent ATP exocytosis is involved in urine storage mechanisms that promote the relaxation of the bladder during the early stages of filling.

Details

Language :
English
ISSN :
2045-2322
Volume :
6
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
27412485
Full Text :
https://doi.org/10.1038/srep29761