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Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings.
- Source :
-
Biomedical materials (Bristol, England) [Biomed Mater] 2016 Jul 07; Vol. 11 (4), pp. 045003. Date of Electronic Publication: 2016 Jul 07. - Publication Year :
- 2016
-
Abstract
- Among the very large number of polymeric materials that have been proposed in the field of orthopedics, polyethylene terephthalate (PET) is one of the most attractive thanks to its flexibility, thermal resistance, mechanical strength and durability. Several studies have been proposed that interface nano- or micro-structured surfaces with mesenchymal stromal cells (MSCs), demonstrating the potential of this technology for promoting osteogenesis. All these studies were carried out on biomaterials other than PET, which remains almost uninvestigated in terms of cell shaping, alignment and differentiation. Here, we study the effect of PET 350-depth nanogratings (NGs) with a ridge and lateral groove size of 500 nm (T1) or 1 μm (T2), on bone marrow-derived human MSC (hMSC) differentiation in relation to the osteogenic fate. We demonstrate that these substrates, especially T2, can promote the osteogenic phenotype more efficiently than standard flat surfaces and that this effect is more marked if cells are cultured in osteogenic medium than in basal medium. Finally, we show that the shape and disposition of calcium hydroxyapatite granules on the different substrates was influenced by the substrate symmetry, being more elongated and spatially organized on NGs than on flat surfaces.
- Subjects :
- Bone Marrow Cells metabolism
Cells, Cultured
Durapatite
Humans
Mesenchymal Stem Cells metabolism
Osteoblasts metabolism
Polyethylene Terephthalates chemistry
Tissue Scaffolds
Bone Marrow Cells cytology
Cell Differentiation
Mesenchymal Stem Cells cytology
Nanostructures chemistry
Osteoblasts cytology
Osteogenesis physiology
Polyethylene Terephthalates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1748-605X
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biomedical materials (Bristol, England)
- Publication Type :
- Academic Journal
- Accession number :
- 27388559
- Full Text :
- https://doi.org/10.1088/1748-6041/11/4/045003