Back to Search
Start Over
Bacterial partition complexes segregate within the volume of the nucleoid.
- Source :
-
Nature communications [Nat Commun] 2016 Jul 05; Vol. 7, pp. 12107. Date of Electronic Publication: 2016 Jul 05. - Publication Year :
- 2016
-
Abstract
- Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria and archaea, this process is assured by a broadly conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex. Competing observations and models imply starkly different 3D localization patterns of the components of the partition machinery during segregation. Here we use super-resolution microscopies to localize in 3D each component of the segregation apparatus with respect to the bacterial chromosome. We show that Par proteins locate within the nucleoid volume and reveal that proper volumetric localization and segregation of partition complexes requires ATPase and DNA-binding activities of ParA. Finally, we find that the localization patterns of the different components of the partition system highly correlate with dense chromosomal regions. We propose a new mechanism in which the nucleoid provides a scaffold to guide the proper segregation of partition complexes.
- Subjects :
- Bacillus subtilis metabolism
Bacillus subtilis ultrastructure
Bacterial Proteins metabolism
Cell Compartmentation genetics
Chromosomes, Bacterial chemistry
Chromosomes, Bacterial metabolism
DNA Primase metabolism
DNA, Bacterial metabolism
Escherichia coli metabolism
Escherichia coli ultrastructure
Escherichia coli Proteins metabolism
Gene Expression
Protein Binding
Bacillus subtilis genetics
Bacterial Proteins genetics
Chromosome Segregation
DNA Primase genetics
DNA, Bacterial genetics
Escherichia coli genetics
Escherichia coli Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 27377966
- Full Text :
- https://doi.org/10.1038/ncomms12107