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Somatic and dendritic perforated-patch recordings reveal b-adrenergic receptor-induced depolarization in medial prefrontal cortex pyramidal neurons.
- Source :
-
Acta neurobiologiae experimentalis [Acta Neurobiol Exp (Wars)] 2016; Vol. 76 (2), pp. 158-64. - Publication Year :
- 2016
-
Abstract
- The aim of this perforated-patch study was to test the effect of isoproterenol on the membrane potential in mPFC (medial prefrontal cortex) pyramidal neurons. Isoproterenol depolarized the membrane potential recorded from the soma. This effect was absent in the presence of metoprolol, suggesting the involvement of beta1-adrenergic receptors. The adenylate cyclase activator forskolin also depolarized the membrane potential. Moreover, the effect of isoproterenol was abolished by the adenylate cyclase inhibitor SQ 22536. This suggested that adenylate cyclase was involved in mediating the effect of the beta-adrenergic receptor agonist. The isoproterenol-induced depolarization persisted after inhibition of protein kinase A with H-89. The effect of beta-adrenergic receptor activation on the membrane potential was dependent on Ih channels because it was abolished in the presence of the Ih channel inhibitor ZD 7288. Dendritic recordings were also performed. In the dendritic segments between 100 microm and 150 microm from the soma and between 200 microm and 250 microm from the soma, isoproterenol also depolarized the membrane potential. The magnitude of the beta-adrenergic receptor-stimulated depolarization was the same in the soma and in both dendritic localizations. The depolarization exerted by isoproterenol may influence PFC cognitive functions.
- Subjects :
- Adenine analogs & derivatives
Adenine pharmacology
Adenylyl Cyclase Inhibitors pharmacology
Adrenergic beta-1 Receptor Antagonists pharmacology
Adrenergic beta-Agonists pharmacology
Animals
Biophysics
Bronchodilator Agents pharmacology
Colforsin pharmacology
Dendrites drug effects
Dose-Response Relationship, Drug
Drug Interactions
Electric Stimulation
In Vitro Techniques
Isoproterenol pharmacology
Male
Membrane Potentials drug effects
Metoprolol pharmacology
Patch-Clamp Techniques
Protein Kinase Inhibitors pharmacology
Rats
Sodium Channel Blockers pharmacology
Tetrodotoxin pharmacology
Dendrites physiology
Membrane Potentials physiology
Prefrontal Cortex cytology
Pyramidal Cells cytology
Pyramidal Cells drug effects
Receptors, Adrenergic, beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1689-0035
- Volume :
- 76
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Acta neurobiologiae experimentalis
- Publication Type :
- Academic Journal
- Accession number :
- 27373953
- Full Text :
- https://doi.org/10.21307/ane-2017-015