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Optimization of a single phase method for lipid extraction from milk.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2016 Aug 05; Vol. 1458, pp. 145-9. Date of Electronic Publication: 2016 Jun 17. - Publication Year :
- 2016
-
Abstract
- For LC-MS-based lipidomic analysis of milk, total lipid extraction from raw milk is generally conducted with Folch or Bligh and Dyer methods; both methods are based on two-phase partition of lipids, and thus time-consuming. In this work, three solvent systems for one-phase extraction of milk lipids were compared with the standard Folch method. Two of the solvent systems (butanol/methanol, 3:1 and 1:1) previously tested for lipid extraction from plasma were found to provide adequate extraction for polar lipids, but incomplete extraction for triglycerides, especially highly lipophilic species. By contrast, our newly designed solvent mix composed of butanol, methanol and chloroform (at a 3:5:4 ratio) provided similar extraction efficiency for triglycerides and higher yield for some of the phospholipids, as compared to the Folch method. This new one-phase extraction method is very simple yet comprehensive and thus suitable for high throughput lipid analysis of milk samples.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Butanols chemistry
Chloroform chemistry
Chromatography, Liquid
Methanol chemistry
Phospholipids chemistry
Phospholipids isolation & purification
Solvents chemistry
Triglycerides chemistry
Triglycerides isolation & purification
Lipids chemistry
Lipids isolation & purification
Mass Spectrometry methods
Milk chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1458
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 27372415
- Full Text :
- https://doi.org/10.1016/j.chroma.2016.06.055