A convenient purification method for silver nanoclusters and its applications in fluorescent pH sensors for bacterial monitoring.

Authors :: Xiong H
Zheng H
Wang W
Liang J
Wen W
Zhang X
Wang S
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2016 Dec 15; Vol. 86, pp. 164-168. Date of Electronic Publication: 2016 Jun 15.
Publication Year :: 2016
Abstract: Herein, we report a convenient approach to purify water-soluble dihydrolipoic acid (DHLA)-capped Ag nanoclusters (Ag NCs) by pH-induced precipitation under acidic conditions. The fluorescence of Ag NCs could be completely recovered by re-dispersing the precipitate into a basic solution using DHLA and NaBH4 as stabilizing ligands and etching reagent. DHLA-Ag NCs-doped agarose hydrogels have been prepared to monitor pH with a wide range from 8.0 to 4.0. When pH decreased, the fluorescence of the hydrogels under a UV lamp decreased and completely disappeared after pH 5. The DHLA-Ag NCs-doped agarose hydrogels biosensor showed low cytotoxicity and long stability. Accordingly, a fluorescent pH sensor for bacterial monitoring has been employed based on the "OFF-ON" signal switch of the Ag NCs-agarose hydrogel.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)

Subjects :: Equipment Design
Equipment Failure Analysis
Fluorescent Dyes chemical synthesis
Fluorescent Dyes isolation & purification
Fractional Precipitation methods
Hydrogen-Ion Concentration
Reproducibility of Results
Sensitivity and Specificity
Silver chemistry
Spectrometry, Fluorescence methods
Thioctic Acid analogs & derivatives
Thioctic Acid chemistry
Bacterial Typing Techniques instrumentation
Colorimetry instrumentation
Escherichia coli K12 isolation & purification
Luminescent Measurements instrumentation
Metal Nanoparticles chemistry
Silver isolation & purification

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