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Diverse distribution of Toxin-Antitoxin II systems in Salmonella enterica serovars.
- Source :
-
Scientific reports [Sci Rep] 2016 Jun 30; Vol. 6, pp. 28759. Date of Electronic Publication: 2016 Jun 30. - Publication Year :
- 2016
-
Abstract
- Type II Toxin-Antitoxin systems (TAs), known for their presence in virulent and antibiotic resistant bacterial strains, were recently identified in Salmonella enterica isolates. However, the relationships between the presence of TAs (ccdAB and vapBC) and the epidemiological and genetic features of different non-typhoidal Salmonella serovars are largely unknown, reducing our understanding of the ecological success of different serovars. Salmonella enterica isolates from different sources, belonging to different serovars and epidemiologically unrelated according to ERIC profiles, were investigated for the presence of type II TAs, plasmid content, and antibiotic resistance. The results showed the ubiquitous presence of the vapBC gene in all the investigated Salmonella isolates, but a diverse distribution of ccdAB, which was detected in the most widespread Salmonella serovars, only. Analysis of the plasmid toxin ccdB translated sequence of four selected Salmonella isolates showed the presence of the amino acid substitution R99W, known to impede in vitro the lethal effect of CcdB toxin in the absence of its cognate antitoxin CcdA. These findings suggest a direct role of the TAs in promoting adaptability and persistence of the most prevalent Salmonella serovars, thus implying a wider eco-physiological role for these type II TAs.
- Subjects :
- Animals
Bacterial Proteins genetics
DNA-Binding Proteins genetics
Humans
Membrane Glycoproteins genetics
Plasmids
Polymerase Chain Reaction
Salmonella enterica drug effects
Salmonella enterica pathogenicity
Serogroup
Toxin-Antitoxin Systems genetics
Drug Resistance, Bacterial drug effects
Salmonella enterica genetics
Salmonella enterica isolation & purification
Toxin-Antitoxin Systems physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27357537
- Full Text :
- https://doi.org/10.1038/srep28759