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[Cloning, expression and identification of gametocyte specific protein Pfgdv1 of Plasmodium falciparum].

Authors :
Su PP
Meng LW
Li JY
Tao ZY
Chen Y
Qiao JC
Wu XX
Jin Y
Wang HP
Fang Q
Wang XM
Xia H
Source :
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control [Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi] 2016 Feb; Vol. 28 (1), pp. 34-8.
Publication Year :
2016

Abstract

Objective: To clone a gametocyte specific protein Pfgdv1 of Plasmodium falciparum, express and identify recombinant Pfgdvl protein in vitro.<br />Methods: PCR was performed to amplify Pfgdv1 from P. falciparum DNA which was got from the patient who was infected with P. falciparum, and the PCR product was inserted into pET28a (+) vector. pET28a-Pfgdv1 recombinant plasmid was constructed and transformed into E. coli host BL21 (DE3+). IPTG was used to induce the recombinant Pfgdv1 protein fused with His tag, and the protein was purified by His-NTA affinity chromatography. The recombinant protein was identified by SDS-PAGE and Western blotting.<br />Results: The PCR product of Pfgdv1 gene was about 1.65 kb, meeting the expectation of predicted fragment size. The recombinant protein was about 67 kDa, which could be recognized by His-Tag monoclonal antibody.<br />Conclusion: The Pfgdv1 gene of P. falciparum is successfully cloned, and the recombinant Pfgdv1 protein is expressed, thereby providing an opportunity for further study on transmission blocking vaccine.

Details

Language :
Chinese
ISSN :
1005-6661
Volume :
28
Issue :
1
Database :
MEDLINE
Journal :
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Publication Type :
Academic Journal
Accession number :
27356402