Generation of KCL032 clinical grade human embryonic stem cell line.

Authors :: Miere C
Wood V
Kadeva N
Cornwell G
Codognotto S
Stephenson E
Ilic D
Source :: Stem cell research [Stem Cell Res] 2016 Jan; Vol. 16 (1), pp. 17-9. Date of Electronic Publication: 2015 Nov 26.
Publication Year :: 2016
Abstract: The KCL032 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.<br /> (Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.)