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Molecular cloning and expression analysis of a new lily-type lectin in the rock bream, Oplegnathus fasciatus.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2016 Dec; Vol. 65, pp. 25-30. Date of Electronic Publication: 2016 Jun 21. - Publication Year :
- 2016
-
Abstract
- A new lily-type lectin RbLTL was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In this study, a new lily-type lectin gene (RbLTL) was cloned from rock bream using expressed sequence tag (EST) analysis. The full-length RbLTL cDNA was encoding a 117-amino acid protein. The deduced amino acid sequence of RbLTL contained all of the conserved features crucial for its fundamental structure, including B-lectin domain and three d-mannose binding sites. RbLTL mRNA was predominately expressed in the gills, with reduced expression noted in intestine tissue. Expression analysis of time series sampled fertilized eggs revealed that expression gradually increased 1, 3, 12, and 24 h: However, expression decreased at 36 h. RbLTL expression was differentially up-regulated in rock bream gills challenged with Streptococcus iniae, Edwardsiella tarda and RSIV. Our results revealed that novel rock bream lily-type lectin may be an important molecule involved in pattern recognition and pathogen elimination in the innate immunity of rock bream.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cloning, Molecular
Fish Proteins genetics
Immunity, Innate
Mannose-Binding Lectins genetics
Transcriptome
DNA Virus Infections immunology
Edwardsiella tarda immunology
Enterobacteriaceae Infections immunology
Fish Proteins metabolism
Gills metabolism
Iridoviridae immunology
Mannose-Binding Lectins metabolism
Perciformes immunology
Streptococcal Infections immunology
Streptococcus iniae immunology
Zygote metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 65
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 27345170
- Full Text :
- https://doi.org/10.1016/j.dci.2016.06.014