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Self-assembled organic-inorganic hybrid glucoamylase nanoflowers with enhanced activity and stability.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2016 Nov; Vol. 92, pp. 660-669. Date of Electronic Publication: 2016 Jun 23. - Publication Year :
- 2016
-
Abstract
- An organic-inorganic hybrid glucoamylase nanoflower was prepared in single pot by simple, facile and highly efficient method. The stepwise formation of enzyme-embedded hybrid nanoflowers and influence of experimental parameters viz. pH of solution mixture, enzyme and copper ion concentration on the activity of prepared hybrid nanoflowers were systematically investigated. The self-assembled hybrid glucoamylase nanoflowers were synthesized by mixing aqueous solution of copper sulphate (200mM) with PBS (pH 7.5, 5mM) containing glucoamylase (1mg/mL) in 24h at room temperature. These prepared nanoflowers were further characterized by FT-IR, SEM and XRD. The hybrid nanoflowers exhibited 204% enhanced activity recovery and two folds improvement in thermal stability in terms of half-life (in the range of 50-70°C) with respect to the free form. The hybrid glucoamylase nanoflowers retained 70% residual activity after eight successive cycles indicating their excellent durability. Additionally, the nanoflowers retained up to 91% residual activity upto 25 days of storage. Moreover, the conformational changes occurred in glucoamylase structure after preparing hybrid nanoflowers were evaluated by FT-IR spectroscopy data tools.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Enzyme Activation
Enzyme Stability
Glucan 1,4-alpha-Glucosidase ultrastructure
Kinetics
Nanostructures ultrastructure
Protein Structure, Secondary
Spectroscopy, Fourier Transform Infrared
Temperature
Glucan 1,4-alpha-Glucosidase chemistry
Inorganic Chemicals chemistry
Nanostructures chemistry
Organic Chemicals chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 92
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 27343706
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2016.06.071