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Endophytic cultivable bacterial community obtained from the Paullinia cupana seed in Amazonas and Bahia regions and its antagonistic effects against Colletotrichum gloeosporioides.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2016 Sep; Vol. 98, pp. 16-22. Date of Electronic Publication: 2016 Jun 23. - Publication Year :
- 2016
-
Abstract
- Guarana (Paullinia cupana var. sorbilis) is a plant from the Amazonas region with socio-economic importance. However, guarana production has been increasingly affected by unfavorable conditions resulting from anthracnose, caused by the Colletotrichum fungal genus, which primarily affects mainly the Amazonas region. The aim of the present study was to isolate bacterial endophytes from the seeds of guarana plants obtained from Amazonas region and the Northeast state of Bahia, a region where this disease is not a problem for guarana plantations. The number of bacterial Colony Forming Units (CFU/g seeds) was 2.4 × 10(4) from the Bahia and 2.9 × 10(4) from the Amazonas region. One hundred and two isolated bacteria were evaluated in vitro against the phytopathogenic strain Colletotrichum gloeosporioides L1. These isolates were also analyzed for the enzymatic production of amylase, cellulase, protease, pectinase, lipase and esterase. Approximately 15% of isolates, showing high antagonistic activity, and the production of at least one enzyme were identified through the partial sequencing of 16S rDNA. The genus Bacillus was the most frequently observed, followed by Paenibacillus, Ochrobactrum, Microbacterium and Stenotrophomonas. Proteolytic activity was observed in 24 isolates followed by amylolytic, pectinolytic and cellulolytic activities. No esterase and lipase production was detected. Most of the isolates, showing antagonistic effects against C. gloeosporioides and high enzymatic activities, were isolated from the anthracnose-affected region. A biocontrol method using the endophytes from guarana seeds could be applied in the future, as these bacteria are vertically transferred to guarana seedlings.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacteria genetics
Bacterial Load
Biodiversity
Brazil
Cluster Analysis
Colony Count, Microbial
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Endophytes genetics
Endophytes physiology
Enzymes analysis
Pest Control, Biological methods
Phylogeny
Plant Diseases prevention & control
RNA, Ribosomal, 16S genetics
Seeds microbiology
Sequence Analysis, DNA
Antibiosis
Bacteria classification
Bacteria isolation & purification
Colletotrichum growth & development
Endophytes classification
Endophytes isolation & purification
Paullinia microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 98
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 27343372
- Full Text :
- https://doi.org/10.1016/j.micpath.2016.06.023