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Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference.
- Source :
-
Scientific reports [Sci Rep] 2016 Jun 22; Vol. 6, pp. 27841. Date of Electronic Publication: 2016 Jun 22. - Publication Year :
- 2016
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Abstract
- RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsRNA. The results showed that TRE1 and TRE2 had compensatory function, and the expression of each increased when the other was silenced. The total rate of insects with phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results demonstrated that TRE silencing could affect the regulation of chitin biosynthesis and degradation, causing moulting deformities. Therefore, expression inhibitors of TREs might be effective tools for the control of planthoppers in rice.
- Subjects :
- Animals
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Hemiptera genetics
Insect Proteins genetics
Insect Proteins metabolism
Oryza parasitology
RNA Interference
RNA, Double-Stranded administration & dosage
Sequence Analysis, RNA
Trehalase metabolism
Chitin biosynthesis
Hemiptera enzymology
Hemiptera growth & development
Metabolic Networks and Pathways
Trehalase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27328657
- Full Text :
- https://doi.org/10.1038/srep27841