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Mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) enhances immune response of infectious bursal disease virus vaccine in chickens.
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2016 May 12; Vol. 15 (2). Date of Electronic Publication: 2016 May 12. - Publication Year :
- 2016
-
Abstract
- In this study, the immune response induced by a mixture of polysaccharide and nucleic acid extracted from Bacillus Calmette-Guerin (BCG) was evaluated in chickens inoculated with infectious bursal disease virus (IBDV) vaccine. After the mixture was injected intramuscularly at a dose of 0.075, 0.15 or 0.3 mg·kg(-1)·day(-1) for 3 days, the 14-day-old chickens were inoculated with the attenuated IBDV vaccine via intranasal and ocular routes. The relative weight of bursa of Fabricius (BF) and thymus, the serum IBD antibody titer, the CD4+/CD8+ ratio, and the concentrations of IFN-γ, IL-2 and IL-6 in peripheral blood were investigated on days 5, 15 and 25. The IBD antibody titer in BCG-treated groups was higher than in the negative control and only IBD-vaccinated chickens, indicating that the mixture of BCG can significantly enhance chicken humoral response. CD4+/CD8+ and the secretions of IFN-γ, IL-2 and IL-6 were also clearly increased compared with that in the negative control and IBD-vaccinated chickens, indicating that the mixture can also enhance the cell-mediated immune response. The results also showed that the relative weights of BF and thymus increased after chickens were inoculated with BCG, indicating that the BCG mixture can clearly enhance the immunity of IBD-vaccine and can be expected to be viewed as a candidate for a new type of immune adjuvant.
- Subjects :
- Adjuvants, Immunologic pharmacology
Animals
Antibodies, Viral blood
BCG Vaccine chemistry
BCG Vaccine pharmacology
Birnaviridae Infections immunology
Chickens immunology
Infectious bursal disease virus metabolism
Male
Nucleic Acids isolation & purification
Nucleic Acids pharmacology
Polysaccharides isolation & purification
Polysaccharides pharmacology
Poultry Diseases therapy
Random Allocation
Vaccines, Attenuated immunology
Vaccines, Attenuated pharmacology
Vaccines, DNA administration & dosage
Vaccines, DNA pharmacology
Viral Vaccines immunology
Viral Vaccines pharmacology
BCG Vaccine immunology
Birnaviridae Infections veterinary
Infectious bursal disease virus immunology
Nucleic Acids immunology
Polysaccharides immunology
Poultry Diseases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 15
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 27323008
- Full Text :
- https://doi.org/10.4238/gmr.15025523