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Application of Fluorescence Polarization in HTS Assays.

Authors :
Huang X
Aulabaugh A
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1439, pp. 115-30.
Publication Year :
2016

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

Subjects

Subjects :
Animals
Anisotropy
Binding, Competitive
Fluorescent Dyes metabolism
Humans
Enzyme Assays methods
Fluorescence Polarization methods
Fluorescent Dyes analysis
High-Throughput Screening Assays methods

Details

Language :
English
ISSN :
1940-6029
Volume :
1439
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
27316991
Full Text :
https://doi.org/10.1007/978-1-4939-3673-1_7
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