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Quantitative Assessment of the Effects of Oxidants on Antigen-Antibody Binding In Vitro.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2016; Vol. 2016, pp. 1480463. Date of Electronic Publication: 2016 May 24. - Publication Year :
- 2016
-
Abstract
- Objective. We quantitatively assessed the influence of oxidants on antigen-antibody-binding activity. Methods. We used several immunological detection methods, including precipitation reactions, agglutination reactions, and enzyme immunoassays, to determine antibody activity. The oxidation-reduction potential was measured in order to determine total serum antioxidant capacity. Results. Certain concentrations of oxidants resulted in significant inhibition of antibody activity but had little influence on total serum antioxidant capacity. Conclusions. Oxidants had a significant influence on interactions between antigen and antibody, but minimal effect on the peptide of the antibody molecule.
- Subjects :
- Adult
Agglutination
Antibodies, Viral immunology
Antigen-Antibody Complex chemistry
Antioxidants analysis
Electrochemical Techniques
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Hepatitis B Surface Antigens immunology
Humans
Immunoprecipitation
Male
Oxidants chemistry
Antigen-Antibody Complex metabolism
Oxidants metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2016
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 27313823
- Full Text :
- https://doi.org/10.1155/2016/1480463