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Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein.
- Source :
-
Parasitology [Parasitology] 2016 Sep; Vol. 143 (11), pp. 1369-81. Date of Electronic Publication: 2016 Jun 17. - Publication Year :
- 2016
-
Abstract
- Up to now, parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Hence, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden. In the present study, a monoclonal antibody (MoAb) against recombinant Fasciola gigantica fatty acid binding protein (rFgFABP) has been produced. As well, a reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) has been developed for the detection of circulating FABP in the sera of mice experimentally and cattle naturally infected with F. gigantica. MoAb 3A3 and biotinylated rabbit anti-recombinant FABP antibody were selected due to their high reactivities and specificities. The lower detection limit of sandwich ELISA was 5 pg mL-1, and no cross-reaction with other parasite antigens was observed. This assay could detect F. gigantica infection from day 1 post infection. In experimental mice, the sensitivity, specificity and accuracy of this assay were 93·3, 100 and 98·2%, while in natural cattle they were 96·7, 100 and 99·1%. Hence, this sandwich ELISA method showed high efficiencies and precisions for diagnosis of fasciolosis by F. gigantica.
- Subjects :
- Animals
Antibodies, Helminth blood
Antibodies, Monoclonal immunology
Antigens, Helminth blood
Antigens, Helminth immunology
Cattle parasitology
Cattle Diseases immunology
Cattle Diseases parasitology
Cross Reactions
Fasciola chemistry
Fascioliasis diagnosis
Fascioliasis immunology
Limit of Detection
Mice
Sensitivity and Specificity
Cattle Diseases diagnosis
Enzyme-Linked Immunosorbent Assay
Fasciola immunology
Fascioliasis veterinary
Fatty Acid-Binding Proteins blood
Fatty Acid-Binding Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8161
- Volume :
- 143
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 27312522
- Full Text :
- https://doi.org/10.1017/S0031182016001104