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A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption.
- Source :
-
Anaerobe [Anaerobe] 2016 Aug; Vol. 40, pp. 76-84. Date of Electronic Publication: 2016 Jun 14. - Publication Year :
- 2016
-
Abstract
- The epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient in Brazil that died from severe diarrhea. A polyphasic approach assigned a new PCR-ribotype and PFGE macrorestriction pattern to strain ICC-45, which is toxigenic (tcdA(+), tcdB(+) and ctdB(+)) and classified as ST41 from MLST Clade 2 and toxinotype IXb. Strain ICC-45 encodes for a variant TcdB that induces a distinct CPE in agreement with its toxinotype. Unlike epidemic NAP1/027 strains, which are also classified to MLST Clade 2, strain ICC-45 is susceptible to fluoroquinolones and does not overproduce toxins TcdA and TcdB. However, supernatants from strain ICC-45 and a NAP1/027 strain produced similar expression of pro-inflammatory cytokines, epithelial damage, and oxidative stress response in the mouse ileal loop model. These results highlight inflammation and oxidative stress as common features in the pathogenesis of C. difficile Clade 2 strains. Finally, this work contributes to the description of differences in virulence among various C. difficile strains.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Animals
Anti-Bacterial Agents pharmacology
Bacterial Proteins metabolism
Bacterial Toxins metabolism
Clostridioides difficile classification
Clostridioides difficile drug effects
Clostridioides difficile genetics
Diarrhea complications
Diarrhea microbiology
Disease Models, Animal
Enterocolitis, Pseudomembranous complications
Enterocolitis, Pseudomembranous drug therapy
Enterocolitis, Pseudomembranous microbiology
Female
Gene Expression
Humans
Intestinal Mucosa microbiology
Intestinal Mucosa pathology
Male
Mice
Multilocus Sequence Typing
Neoplasms complications
Neoplasms microbiology
Oxidative Stress
Phylogeny
Polymerase Chain Reaction
Ribotyping
Bacterial Proteins genetics
Bacterial Toxins genetics
Clostridioides difficile isolation & purification
Diarrhea diagnosis
Enterocolitis, Pseudomembranous diagnosis
Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8274
- Volume :
- 40
- Database :
- MEDLINE
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 27311833
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2016.06.005