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Dynamic Filament Formation by a Divergent Bacterial Actin-Like ParM Protein.
- Source :
-
PloS one [PLoS One] 2016 Jun 16; Vol. 11 (6), pp. e0156944. Date of Electronic Publication: 2016 Jun 16 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Actin-like proteins (Alps) are a diverse family of proteins whose genes are abundant in the chromosomes and mobile genetic elements of many bacteria. The low-copy-number staphylococcal multiresistance plasmid pSK41 encodes ParM, an Alp involved in efficient plasmid partitioning. pSK41 ParM has previously been shown to form filaments in vitro that are structurally dissimilar to those formed by other bacterial Alps. The mechanistic implications of these differences are not known. In order to gain insights into the properties and behavior of the pSK41 ParM Alp in vivo, we reconstituted the parMRC system in the ectopic rod-shaped host, E. coli, which is larger and more genetically amenable than the native host, Staphylococcus aureus. Fluorescence microscopy showed a functional fusion protein, ParM-YFP, formed straight filaments in vivo when expressed in isolation. Strikingly, however, in the presence of ParR and parC, ParM-YFP adopted a dramatically different structure, instead forming axial curved filaments. Time-lapse imaging and selective photobleaching experiments revealed that, in the presence of all components of the parMRC system, ParM-YFP filaments were dynamic in nature. Finally, molecular dissection of the parMRC operon revealed that all components of the system are essential for the generation of dynamic filaments.
- Subjects :
- Actins biosynthesis
Bacterial Proteins
Escherichia coli Proteins biosynthesis
Gene Expression Regulation, Bacterial
Luminescent Proteins
Microscopy, Fluorescence
Operon genetics
Optical Imaging
Actin Cytoskeleton genetics
Actins genetics
Escherichia coli Proteins genetics
Plasmids genetics
Staphylococcus aureus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 27310470
- Full Text :
- https://doi.org/10.1371/journal.pone.0156944