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Different bone remodeling levels of trabecular and cortical bone in response to changes in Wnt/β-catenin signaling in mice.
- Source :
-
Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2017 Apr; Vol. 35 (4), pp. 812-819. Date of Electronic Publication: 2016 Jul 20. - Publication Year :
- 2017
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Abstract
- Trabecular bone and cortical bone have different bone remodeling levels, and the underlying mechanisms are not fully understood. In the present study, the expression of Wnt/β-catenin signaling and its downstream molecules along with bone mass in trabecular and cortical bone were compared in wild-type mice, constitutive activation of β-catenin (CA-β-catenin) mice and β-catenin deletion mice. It was found that the expression level of most of the examined genes such as Wnt3a, β-catenin, osteocalcin and RANKL/OPG ratio were significantly higher in trabecular bone than in cortical bone in wild-type mice. CA-β-catenin resulted in up-regulated expression of the above-mentioned genes except for RANKL/OPG ratio, which were down-regulated. Also, CA-β-catenin led to increased number of osteoblasts, decreased number of osteoclasts and increased bone mass in both the trabecular bone and cortical bone compared with wild-type mice; however, the extent of changes was much greater in the trabecular bone than in the cortical bone. By contrast, null β-catenin led to down-regulated expression of the above-mentioned genes except for RANKL/OPG ratio. Furthermore, β-catenin deletion led to decreased number of osteoblasts, increased number of osteoclasts and decreased bone mass when compared with wild-type mice. Again, the extent of these changes was more significant in trabecular bone than cortical bone. Taken together, we found that the expression level of Wnt/β-catenin signaling and bone remodeling-related molecules were different in cortical bone and trabecular bone, and the trabecular bone was more readily affected by changes in the Wnt/β-catenin signaling pathway. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:812-819, 2017.<br /> (© 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)
- Subjects :
- Animals
Bone and Bones metabolism
Gene Expression Regulation
Mice
Mice, Transgenic
Osteoblasts metabolism
Osteoclasts metabolism
RANK Ligand metabolism
Signal Transduction
Wnt Proteins metabolism
X-Ray Microtomography
beta Catenin metabolism
Bone Remodeling
Cancellous Bone physiology
Cortical Bone physiology
Wnt Signaling Pathway
Subjects
Details
- Language :
- English
- ISSN :
- 1554-527X
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society
- Publication Type :
- Academic Journal
- Accession number :
- 27306622
- Full Text :
- https://doi.org/10.1002/jor.23339