Back to Search
Start Over
[Effect of the 10 kb sequence of piscine Streptococcus agalactiae on bacterial virulence].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2016 Jan 04; Vol. 56 (1), pp. 110-9. - Publication Year :
- 2016
-
Abstract
- Objective: From the previous comparative genomic analysis, we found a specific unknown 10 kb sequence (including 11 Open reading Frames) in Chinese piscine strain GD201008-001 genome. To study the role of 10 kb in the pathogenicity of piscine S. agalactiae, the 10 kb sequence was deleted from the GD201008-001 genome.<br />Methods: The isogenic mutant Δ10 kb was constructed by using the temperature-sensitive Streptococcus-E. coli shuttle vector pSET4s. We compared the growth characteristics, adherence to HEp-2 cell and bacterial virulence in a zebrafish infection model between wild strain and mutant. Meanwhile the expressions of the known virulence genes from GD201008-001 and Δ10 kb were also quantified by real-time PCR.<br />Results: The Δ10 kb showed no significant differences in bacterial morphology and adherence to HEp-2 cells compared with the wild-type strain, but the speed of growth was slightly slower than the wild strain. Furthermore the 50% lethal dose of Δ10 kb was decreased up to 10-fold (P < 0.001) of the parental strain in a zebrafish infection model, and the expressions of the virulence genes, PI-2b and neul, were significantly increased in the mutant.<br />Conclusion: These findings demonstrated that the 10 kb sequence of piscine Streptococcus agalactiae exerts a significant effect on bacterial virulence and probably regulates the virulence genes expression of GD20 1008-001.
- Subjects :
- Animals
Bacterial Proteins genetics
Gene Expression Regulation, Bacterial
Streptococcal Infections microbiology
Streptococcus agalactiae genetics
Streptococcus agalactiae metabolism
Virulence
Zebrafish
Bacterial Proteins metabolism
Fish Diseases microbiology
Streptococcal Infections veterinary
Streptococcus agalactiae pathogenicity
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 56
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 27305785