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Intrinsically disordered region of influenza A NP regulates viral genome packaging via interactions with viral RNA and host PI(4,5)P2.
- Source :
-
Virology [Virology] 2016 Sep; Vol. 496, pp. 116-126. Date of Electronic Publication: 2016 Jun 09. - Publication Year :
- 2016
-
Abstract
- To be incorporated into progeny virions, the viral genome must be transported to the inner leaflet of the plasma membrane (PM) and accumulate there. Some viruses utilize lipid components to assemble at the PM. For example, simian virus 40 (SV40) targets the ganglioside GM1 and human immunodeficiency virus type 1 (HIV-1) utilizes phosphatidylinositol (4,5) bisphosphate [PI(4,5)P2]. Recent studies clearly indicate that Rab11-mediated recycling endosomes are required for influenza A virus (IAV) trafficking of vRNPs to the PM but it remains unclear how IAV vRNP localized or accumulate underneath the PM for viral genome incorporation into progeny virions. In this study, we found that the second intrinsically disordered region (IDR2) of NP regulates two binding steps involved in viral genome packaging. First, IDR2 facilitates NP oligomer binding to viral RNA to form vRNP. Secondly, vRNP assemble by interacting with PI(4,5)P2 at the PM via IDR2. These findings suggest that PI(4,5)P2 functions as the determinant of vRNP accumulation at the PM.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Dogs
Host-Pathogen Interactions
Humans
Models, Molecular
Molecular Conformation
Nucleocapsid Proteins
Phosphatidylinositol 4,5-Diphosphate chemistry
Protein Binding
Protein Multimerization
Protein Transport
RNA-Binding Proteins chemistry
Structure-Activity Relationship
Viral Core Proteins chemistry
Virus Replication
Genome, Viral
Influenza A virus physiology
Influenza, Human metabolism
Influenza, Human virology
Intrinsically Disordered Proteins metabolism
Phosphatidylinositol 4,5-Diphosphate metabolism
RNA, Viral chemistry
RNA, Viral metabolism
RNA-Binding Proteins metabolism
Viral Core Proteins metabolism
Virus Assembly
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 496
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 27289560
- Full Text :
- https://doi.org/10.1016/j.virol.2016.05.018