Identification of Lactobacillus brevis using a species-specific AFLP-derived marker.

Authors :: Fusco V
Quero GM
Chieffi D
Franz CM
Source :: International journal of food microbiology [Int J Food Microbiol] 2016 Sep 02; Vol. 232, pp. 90-4. Date of Electronic Publication: 2016 Jun 04.
Publication Year :: 2016
Abstract: A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)

Subjects :: Aldehyde Reductase genetics
Aldo-Keto Reductases
Base Sequence
Bread microbiology
Cheese microbiology
DNA Primers genetics
Fermentation
Levilactobacillus brevis classification
Levilactobacillus brevis enzymology
Meat microbiology
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Species Specificity
Amplified Fragment Length Polymorphism Analysis methods
DNA, Bacterial genetics
Levilactobacillus brevis genetics
Molecular Typing methods
Polymerase Chain Reaction methods

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