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MiR-370 promotes cell death of liver cancer cells by Akt/FoxO3a signalling pathway.

MiR-370 promotes cell death of liver cancer cells by Akt/FoxO3a signalling pathway.

Authors :
Sun G
Hou YB
Jia HY
Bi XH
Yu L
Chen DJ
Source :
European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2016 May; Vol. 20 (10), pp. 2011-9.
Publication Year :
2016

Abstract

Objective: MicroRNAs have emerged as key regulators in cancer cell biology. In the present study, we investigate the role and the involving mechanism of miR-370 in the progression of liver cancers.<br />Materials and Methods: MiR-370 levels were detected by real-time PCR assay. Cell proliferation of HepG2, MHCC-97H and SMMC-7721 was determined by MTT assay. PI staining was detected by FACS analysis. Colony formation was used to test liver cancer cell growth. FoxO3a and Akt expression was determined by western blotting analysis.<br />Results: MiR-370 level was significantly down-regulated in liver cancer cells. Functional analysis revealed that miR-370 mimics suppressed cell proliferation of liver cancer cells, while transfection with miR-370 inhibitor increased cell proliferation of liver cancer cells. Moreover, miR-370 mimics induced cell death of HepG2. Furthermore, Western blotting analysis results demonstrated that miR-370 inhibited the proliferation of liver cancer cells by activating FoxO3a.<br />Conclusions: MiR-370 inhibited cell proliferation of liver cancer cells by PI3K/Akt signaling pathway. It worked as a tumor suppressor to suppress the progression of human liver cancers.

Details

Language :
English
ISSN :
2284-0729
Volume :
20
Issue :
10
Database :
MEDLINE
Journal :
European review for medical and pharmacological sciences
Publication Type :
Academic Journal
Accession number :
27249599