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Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Jul 25; Vol. 55 (31), pp. 9041-4. Date of Electronic Publication: 2016 May 30. - Publication Year :
- 2016
-
Abstract
- The pretreatment of cultured pheochromocytoma (PC12) cells with cis-diamminedichloroplatinum (cisplatin), an anti-cancer drug, influences the exocytotic ability of the cells in a dose-dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single-cell amperometry reflects that 2 μm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 μm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.<br /> (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Catecholamines antagonists & inhibitors
Catecholamines chemistry
Dose-Response Relationship, Drug
Neurotransmitter Agents antagonists & inhibitors
Neurotransmitter Agents chemistry
PC12 Cells
Rats
Tumor Cells, Cultured
Antineoplastic Agents pharmacology
Catecholamines metabolism
Cisplatin pharmacology
Exocytosis drug effects
Neurotransmitter Agents metabolism
Single-Cell Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 55
- Issue :
- 31
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 27239950
- Full Text :
- https://doi.org/10.1002/anie.201602977