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Estrogen modulation of calretinin and BDNF expression in midbrain dopaminergic neurons of ovariectomised mice.
- Source :
-
Journal of chemical neuroanatomy [J Chem Neuroanat] 2016 Nov; Vol. 77, pp. 60-67. Date of Electronic Publication: 2016 May 19. - Publication Year :
- 2016
-
Abstract
- Estrogen attenuates the loss of dopamine neurons from the substantia nigra in animal models of Parkinson's disease (PD) and excitatory amino-acid induced neurotoxicity by interactions with brain-derived neurotrophic factor (BDNF), and calretinin (CR) containing dopaminergic (DA) neurons. To examine this interaction more closely, we treated the ovariectomised (OVX) mice with estrodial for 10days, and compared these mice to those OVX mice injected with the vehicle or control mice. Estrogen treatment in OVX mice had significantly more tyrosine hydroxylase (TH) positive neurons in the substantia nigra pars compacta (SNpc). Dopamine transporter (DAT) mRNA and BDNF mRNA levels in the midbrain were also significantly increased by estrogen treatment (P<0.05). OVX markedly decreased the number of TH/CR double stained cells in the SNpc (P<0.05), a trend which could be reversed by estrogen treatment. However, the number of GFAP positive cells in the substantia nigra did not show significant changes (P >0.05) after vehicle or estrodial treatment. Furthermore, we found that estrogen treatment abrogated the OVX-induced decrease in the phosphorylated AKT (p-AKT), but not p-ERK. We hypothesize that short-term treatment with estrogen confers neuroprotection to DA neurons by increasing CR in the DA neurons and BDNF in the midbrain, which possibly related to activation of the PI3K/Akt signaling pathway.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Astrocytes drug effects
Astrocytes metabolism
Brain-Derived Neurotrophic Factor genetics
Calbindin 2 genetics
Dopamine Plasma Membrane Transport Proteins biosynthesis
Dopamine Plasma Membrane Transport Proteins genetics
Dopaminergic Neurons drug effects
Female
Glial Fibrillary Acidic Protein metabolism
MAP Kinase Signaling System drug effects
MAP Kinase Signaling System genetics
Mesencephalon cytology
Mesencephalon drug effects
Mice
Mice, Inbred C57BL
Oncogene Protein v-akt biosynthesis
Oncogene Protein v-akt genetics
Ovariectomy
Substantia Nigra drug effects
Substantia Nigra enzymology
Substantia Nigra metabolism
Tyrosine 3-Monooxygenase metabolism
Brain-Derived Neurotrophic Factor biosynthesis
Calbindin 2 biosynthesis
Dopaminergic Neurons metabolism
Estradiol pharmacology
Mesencephalon metabolism
Neuroprotective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6300
- Volume :
- 77
- Database :
- MEDLINE
- Journal :
- Journal of chemical neuroanatomy
- Publication Type :
- Academic Journal
- Accession number :
- 27211874
- Full Text :
- https://doi.org/10.1016/j.jchemneu.2016.05.005