Back to Search
Start Over
A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency.
- Source :
-
Plant physiology [Plant Physiol] 2016 Jul; Vol. 171 (3), pp. 2017-27. Date of Electronic Publication: 2016 May 19. - Publication Year :
- 2016
-
Abstract
- Iron (Fe) deficiency affects plant growth and development, leading to reduction of crop yields and quality. Although the regulation of Fe uptake under Fe deficiency has been well studied in the past decade, the regulatory mechanism of Fe translocation inside the plants remains unknown. Here, we show that a WRKY transcription factor WRKY46 is involved in response to Fe deficiency. Lack of WRKY46 (wrky46-1 and wrky46-2 loss-of-function mutants) significantly affects Fe translocation from root to shoot and thus causes obvious chlorosis on the new leaves under Fe deficiency. Gene expression analysis reveals that expression of a nodulin-like gene (VACUOLAR IRON TRANSPORTER1-LIKE1 [VITL1]) is dramatically increased in wrky46-1 mutant. VITL1 expression is inhibited by Fe deficiency, while the expression of WRKY46 is induced in the root stele. Moreover, down-regulation of VITL1 expression can restore the chlorosis phenotype on wrky46-1 under Fe deficiency. Further yeast one-hybrid and chromatin immunoprecipitation experiments indicate that WRKY46 is capable of binding to the specific W-boxes present in the VITL1 promoter. In summary, our results demonstrate that WRKY46 plays an important role in the control of root-to-shoot Fe translocation under Fe deficiency condition via direct regulation of VITL1 transcript levels.<br /> (© 2016 American Society of Plant Biologists. All Rights Reserved.)
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Basic Helix-Loop-Helix Transcription Factors metabolism
Biological Transport
Gene Expression Regulation, Plant
Mutation
Plant Roots genetics
Plant Roots metabolism
Plant Shoots genetics
Plant Shoots metabolism
Plants, Genetically Modified
Promoter Regions, Genetic
Transcription Factors genetics
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Iron metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 171
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 27208259
- Full Text :
- https://doi.org/10.1104/pp.16.00252