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Host Immune Responses Differ between M. africanum- and M. tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment.
- Source :
-
PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2016 May 18; Vol. 10 (5), pp. e0004701. Date of Electronic Publication: 2016 May 18 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb- compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1α were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-γ and TNF-α production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb- compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility.
- Subjects :
- Adolescent
Adult
Aged
Antigens, Bacterial immunology
Biomarkers blood
Female
Gambia
Gene Expression
Host-Pathogen Interactions
Humans
Interferon-gamma blood
Interleukin-5 blood
Interleukin-8 blood
Male
Middle Aged
Mycobacterium drug effects
Mycobacterium isolation & purification
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis isolation & purification
Tuberculosis ethnology
Tuberculosis microbiology
Tumor Necrosis Factor-alpha drug effects
Young Adult
Antitubercular Agents therapeutic use
Cytokines blood
Mycobacterium immunology
Mycobacterium tuberculosis immunology
Tuberculosis drug therapy
Tuberculosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1935-2735
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PLoS neglected tropical diseases
- Publication Type :
- Academic Journal
- Accession number :
- 27192147
- Full Text :
- https://doi.org/10.1371/journal.pntd.0004701