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Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections.
- Source :
-
Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2016 Aug; Vol. 241 (14), pp. 1551-8. Date of Electronic Publication: 2016 May 13. - Publication Year :
- 2016
-
Abstract
- We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.<br /> (© 2016 by the Society for Experimental Biology and Medicine.)
- Subjects :
- Acute Disease
Adolescent
Adult
Aged
Aged, 80 and over
DNA, Bacterial chemistry
DNA, Bacterial isolation & purification
DNA, Viral chemistry
DNA, Viral isolation & purification
Female
Humans
Male
Middle Aged
Polymerase Chain Reaction methods
Respiratory Tract Infections microbiology
Respiratory Tract Infections virology
Sensitivity and Specificity
Diagnostic Techniques, Respiratory System
Oligonucleotide Array Sequence Analysis methods
Respiratory Tract Infections diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3699
- Volume :
- 241
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Experimental biology and medicine (Maywood, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27190247
- Full Text :
- https://doi.org/10.1177/1535370216647128