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A new lactobacilli in vivo expression system for the production and delivery of heterologous proteins at mucosal surfaces.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2016 Jul; Vol. 363 (13). Date of Electronic Publication: 2016 May 01. - Publication Year :
- 2016
-
Abstract
- Food-grade lactic acid bacteria, such as lactobacilli, represent good candidates for the development of mucosal vectors. Indeed, they are generally recognized as safe microorganisms and some strains display beneficial effects (probiotics). In this study, we described a new lactobacilli in vivo expression (LIVE) system for the production and delivery of therapeutic molecules at mucosal surfaces. The versatility and functionality of this system was successfully validated in several lactobacilli species; furthermore, we assessed in vivo LIVE system in two different mouse models of human pathologies: (i) a model of therapy against intestinal inflammation (inflammatory bowel diseases) and (ii) a model of vaccination against dental caries. We demonstrated that Lactobacillus gasseri expressing the anti-inflammatory cytokine IL-10 under LIVE system efficiently delivered the recombinant protein at mucosal surfaces and display anti-inflammatory effects. In the vaccination model against caries, LIVE system allowed the heterologous expression of Streptococcus mutans antigen GbpB by L. gasseri, leading to a stimulation of the host immune response.<br /> (© FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
Dental Caries prevention & control
Gene Expression
Glycoproteins genetics
Humans
Inflammation
Inflammatory Bowel Diseases drug therapy
Interleukin-10 administration & dosage
Interleukin-10 genetics
Mice
Models, Animal
Mucous Membrane drug effects
Recombinant Proteins genetics
Recombinant Proteins therapeutic use
Streptococcus mutans chemistry
Vaccination
Antigens, Bacterial immunology
Antigens, Bacterial metabolism
Bacterial Proteins immunology
Bacterial Proteins metabolism
Genetic Vectors
Glycoproteins immunology
Glycoproteins metabolism
Interleukin-10 metabolism
Interleukin-10 therapeutic use
Lactobacillus gasseri genetics
Mucous Membrane metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1574-6968
- Volume :
- 363
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 27190148
- Full Text :
- https://doi.org/10.1093/femsle/fnw117