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Simultaneous photoelectrochemical and visualized immunoassay of β-human chorionic gonadotrophin.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2016 Nov 15; Vol. 85, pp. 294-299. Date of Electronic Publication: 2016 May 06. - Publication Year :
- 2016
-
Abstract
- Herein, on the basis of the alkaline phosphate (ALP) induced reaction, a simultaneous photoelectrochemical (PEC) and visualized immunoassay has been established for the detection of β-human chorionic gonadotrophin (β-HCG). Specifically, in the proposed system, ALP stimulated the oxidative hydrolyzing transformation of 5-bromo-4-chloro-3-indoyl phosphate (BCIP) to an indigo precipitation, generating an insulating layer that impeded the interfacial mass and electron transfer and thus the photocurrent production. Meanwhile, a visualized detection could be performed according to the change of color intensity. Upon proper experimental conditions, the protocol possessed a detection range of 0.5-1000IU/L with a detection limit of (0.20±0.011)IU/L toward β-HCG. With high sensitivity and specificity, this work presents the first general protocol for simultaneous PEC and visualized detection, which could be easily extended to addressing numerous other targets.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Alkaline Phosphatase chemistry
Antibodies, Immobilized chemistry
Biosensing Techniques methods
Cadmium Compounds chemistry
Colorimetry methods
Electrodes
Humans
Quantum Dots chemistry
Quantum Dots ultrastructure
Selenium Compounds chemistry
Chorionic Gonadotropin analysis
Electrochemical Techniques methods
Immunoassay methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 85
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 27183279
- Full Text :
- https://doi.org/10.1016/j.bios.2016.04.103