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Lack of strong anti-viral immune gene stimulation in Torque Teno Sus Virus1 infected macrophage cells.
- Source :
-
Virology [Virology] 2016 Aug; Vol. 495, pp. 63-70. Date of Electronic Publication: 2016 May 11. - Publication Year :
- 2016
-
Abstract
- While recent findings suggest that swine TTVs (TTSuVs) can act as primary or co-infecting pathogens, very little is known about viral immunity. To determine whether TTSuVs downregulate key host immune responses to facilitate their own survival, a swine macrophage cell line, 3D4/31, was used to over-express recombinant TTSuV1 viral particles or the ORF3 protein. Immune gene expression profiles were assessed by a quantitative PCR panel consisting of 22 immune genes, in cell samples collected at 6, 12, 24 and 48h post-transfection. Despite the upregulation of IFN-β and TLR9, interferon stimulated innate genes and pro-inflammatory genes were not upregulated in virally infected cells. The adaptive immune genes, IL-4 and IL-13, were significantly downregulated at 6h post-transfection. The ORF3 protein did not appear do not have a major immuno-suppressive effect, nor did it stimulate anti-viral immunity. Data from this study warrants further investigation into the mechanisms of TTV related immuno-pathogenesis.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Adaptive Immunity genetics
Animals
Cell Line
Cytokines genetics
Cytokines metabolism
DNA Virus Infections metabolism
DNA Virus Infections virology
Disease Resistance genetics
Disease Resistance immunology
Genome, Viral
Immunomodulation genetics
Inflammation Mediators metabolism
Macrophages metabolism
Macrophages virology
Open Reading Frames
Swine
Swine Diseases genetics
Swine Diseases immunology
Swine Diseases virology
Time Factors
Torque teno virus pathogenicity
DNA Virus Infections genetics
DNA Virus Infections immunology
Host-Pathogen Interactions genetics
Host-Pathogen Interactions immunology
Immunity, Innate genetics
Macrophages immunology
Torque teno virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 495
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 27179346
- Full Text :
- https://doi.org/10.1016/j.virol.2016.04.028