Back to Search
Start Over
Development of a rapid diagnostic assay based on magnetic bead purification of OXA-β-lactamase mRNA.
- Source :
-
Future microbiology [Future Microbiol] 2016 May; Vol. 11, pp. 617-29. Date of Electronic Publication: 2016 May 10. - Publication Year :
- 2016
-
Abstract
- Aim: To countermeasure the global spread of β-lactamases, we developed a rapid molecular test for the highly variable OXA-β-lactamases that allows minimizing the time to effective treatment.<br />Methods: OXA-mRNA was specifically enriched from total RNA using group-specific biotinylated DNA probes and streptavidin-coated magnetic beads. Phylogenetic OXA groups were distinguished by PCR product size.<br />Results: This mRNA fishing method is highly sensitive, yielding specific results from as little as 1 ng total RNA. It enables discrimination of OXA-extended substrate spectrum β-lactamases and carbapenemases and the semi-quantitative detection of highly expressed ISAba1-controlled variants.<br />Conclusion: Targeting mRNA with specific probes on magnetic beads will allow for adaptation to automated systems, such as point-of-care diagnostics.
- Subjects :
- Acinetobacter Infections microbiology
Acinetobacter baumannii classification
Acinetobacter baumannii genetics
Acinetobacter baumannii isolation & purification
Bacterial Proteins metabolism
Enterobacteriaceae classification
Enterobacteriaceae genetics
Enterobacteriaceae isolation & purification
Enterobacteriaceae Infections microbiology
Humans
Magnetics instrumentation
Phylogeny
RNA, Bacterial genetics
RNA, Bacterial metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
beta-Lactamases metabolism
Acinetobacter Infections diagnosis
Acinetobacter baumannii enzymology
Bacterial Proteins genetics
Enterobacteriaceae enzymology
Enterobacteriaceae Infections diagnosis
Magnetics methods
RNA, Bacterial isolation & purification
RNA, Messenger isolation & purification
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1746-0921
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Future microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27164315
- Full Text :
- https://doi.org/10.2217/fmb.16.13