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Differential expression of microRNAs and potential targets under drought stress in barley.
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2017 Jan; Vol. 40 (1), pp. 11-24. Date of Electronic Publication: 2016 Aug 04. - Publication Year :
- 2017
-
Abstract
- Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.<br /> (© 2016 The Authors Plant, Cell & Environment Published by John Wiley & Sons Ltd.)
- Subjects :
- Base Sequence
Gene Expression Regulation, Plant
Genes, Plant
Genotype
Hordeum cytology
MicroRNAs metabolism
Plant Leaves cytology
Plant Leaves genetics
RNA Stability genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Droughts
Gene Expression Profiling
Hordeum genetics
Hordeum physiology
MicroRNAs genetics
Stress, Physiological genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 40
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 27155357
- Full Text :
- https://doi.org/10.1111/pce.12764