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Differential expression of microRNAs and potential targets under drought stress in barley.

Authors :
Ferdous J
Sanchez-Ferrero JC
Langridge P
Milne L
Chowdhury J
Brien C
Tricker PJ
Source :
Plant, cell & environment [Plant Cell Environ] 2017 Jan; Vol. 40 (1), pp. 11-24. Date of Electronic Publication: 2016 Aug 04.
Publication Year :
2017

Abstract

Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.<br /> (© 2016 The Authors Plant, Cell & Environment Published by John Wiley & Sons Ltd.)

Details

Language :
English
ISSN :
1365-3040
Volume :
40
Issue :
1
Database :
MEDLINE
Journal :
Plant, cell & environment
Publication Type :
Academic Journal
Accession number :
27155357
Full Text :
https://doi.org/10.1111/pce.12764