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Chromosome identification for the carnivorous plant Genlisea margaretae.

Authors :
Tran TD
Šimková H
Schmidt R
Doležel J
Schubert I
Fuchs J
Source :
Chromosoma [Chromosoma] 2017 Jun; Vol. 126 (3), pp. 389-397. Date of Electronic Publication: 2016 May 07.
Publication Year :
2017

Abstract

Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.

Details

Language :
English
ISSN :
1432-0886
Volume :
126
Issue :
3
Database :
MEDLINE
Journal :
Chromosoma
Publication Type :
Academic Journal
Accession number :
27153834
Full Text :
https://doi.org/10.1007/s00412-016-0599-0