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Role of endothelin receptors and relationship with nitric oxide synthase in impaired erectile response in diabetic rats.
- Source :
-
Andrologia [Andrologia] 2017 Mar; Vol. 49 (2). Date of Electronic Publication: 2016 May 04. - Publication Year :
- 2017
-
Abstract
- To evaluate the protective role of bosentan (BOS), an endothelin-1 (ET-1) receptor antagonist, and to show the changes in rats with experimentally induced diabetic erectile dysfunction (ED), a total of 24 albino Wistar rats were allocated into four groups. Group 1 was the healthy group and Group 2 had diabetes mellitus (DM) induced by intraperitoneal injection of 60 mg kg <superscript>-1</superscript> streptozotocin (STZ). Following the establishment of DM, Group 3 and Group 4 were treated with oral BOS doses of 50 mg kg <superscript>-1</superscript> and 100 mg kg <superscript>-1</superscript> , respectively, for 60 days. At the end of the treatment, we evaluated yawning and erection response to apomorphine treatment and then the animals were sacrificed. ET-1, eNOS, iNOS, tumour necrosis factor (TNF)-α, ET-RA and ET-RB mRNA expressions were analysed in cavernosal tissue. It was observed that yawning and erection response decreased in the diabetic group; however, both of these improved with BOS treatment. While ET-1, TNF-α and iNOS gene expressions increased, eNOS, ET-RA and ET-RB gene expressions decreased in the DM group compared to the healthy group. DM has a negative impact on cavernosal tissue blood flow through activating vasoconstrictor mediators in cavernosal tissue. BOS regulates significantly eNOS, iNOS and TNF-α expressions in a dose-dependent manner.<br /> (© 2016 Blackwell Verlag GmbH.)
- Subjects :
- Animals
Apomorphine pharmacology
Bosentan
Dopamine Agonists pharmacology
Endothelin Receptor Antagonists administration & dosage
Endothelin-1 metabolism
Humans
Male
Nitric Oxide metabolism
Nitric Oxide Synthase Type II metabolism
Nitric Oxide Synthase Type III metabolism
Penis blood supply
Penis metabolism
RNA, Messenger metabolism
Rats
Rats, Wistar
Receptor, Endothelin A metabolism
Receptor, Endothelin B metabolism
Sulfonamides administration & dosage
Tumor Necrosis Factor-alpha metabolism
Diabetes Mellitus, Experimental complications
Endothelin Receptor Antagonists therapeutic use
Erectile Dysfunction drug therapy
Erectile Dysfunction etiology
Penile Erection drug effects
Sulfonamides therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0272
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Andrologia
- Publication Type :
- Academic Journal
- Accession number :
- 27145076
- Full Text :
- https://doi.org/10.1111/and.12607