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Sweeping of hydrophobic amines under inhomogeneous electric field and low surfactant concentration in micellar electrokinetic chromatography.
- Source :
-
Electrophoresis [Electrophoresis] 2016 May; Vol. 37 (9), pp. 1161-5. Date of Electronic Publication: 2015 Dec 09. - Publication Year :
- 2016
-
Abstract
- The influence of sample matrix on sample sweeping in MEKC was examined in the presented manuscript. Significant focusing effect was observed for relatively hydrophobic cationic compounds (emetine, strychnine and quinine) using high ionic strength sample matrix (900 mM H3 PO4 /720 mM Tris) which conductivity was about ninefold higher than utilized BGE. Moreover, the results were obtained using BGE composed of comparatively low surfactant concentration (10 mM SDS) and 40 mM H3 PO4 /32 mM Tris buffer solution. About 200 to 300-fold preconcentration of analytes was reached with the presented method. Basing on experimental results and computer simulation using Simul5 software, hypothetical mechanism of observed phenomenon was proposed.<br /> (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Computer Simulation
Emetine analysis
Emetine chemistry
Emetine isolation & purification
Hydrophobic and Hydrophilic Interactions
Models, Chemical
Quinine analysis
Quinine chemistry
Quinine isolation & purification
Strychnine analysis
Strychnine chemistry
Strychnine isolation & purification
Chromatography, Micellar Electrokinetic Capillary methods
Surface-Active Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1522-2683
- Volume :
- 37
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Electrophoresis
- Publication Type :
- Academic Journal
- Accession number :
- 27135308
- Full Text :
- https://doi.org/10.1002/elps.201500436