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Autologous chondrocytes as a novel source for neo-chondrogenesis in haemophiliacs.
- Source :
-
Cell and tissue research [Cell Tissue Res] 2016 Oct; Vol. 366 (1), pp. 51-61. Date of Electronic Publication: 2016 Apr 29. - Publication Year :
- 2016
-
Abstract
- Haemophilic arthropathy is the major cause of disability in patients with haemophilia and, despite prophylaxis with coagulation factor concentrates, some patients still develop articular complications. We evaluate the feasibility of a tissue engineering approach to improve current clinical strategies for cartilage regeneration in haemophiliacs by using autologous chondrocytes (haemophilic chondrocytes; HaeCs). Little is known about articular chondrocytes from haemophilic patients and no characterisation has as yet been performed. An investigation into whether blood exposure alters HaeCs should be interesting from the perspective of autologous implants. The typical morphology and expression of specific target genes and surface markers were therefore assessed by optical microscopy, reverse transcription plus the polymerase chain reaction (PCR), real-time PCR and flow-cytometry. We then considered chondrocyte behaviour on a bio-hybrid scaffold (based on polyvinyl alcohol/Wharton's jelly) as an in vitro model of articular cartilage prosthesis. Articular chondrocytes from non-haemophilic donors were used as controls. HaeC morphology and the resulting immunophenotype CD44(+)/CD49c(+)/CD49e(+)/CD151(+)/CD73(+)/CD49f(-)/CD26(-) resembled those of healthy donors. Moreover, HaeCs were active in the transcription of genes involved in the synthesis of the extracellular matrix proteins of the articular cartilage (ACAN, COL1A, COL2A, COL10A, COL9A, COMP, HAS1, SOX9), although the over-expression of COL1A1, COL10A1, COMP and HAS was observed. In parallel, the composite scaffold showed adequate mechanical and biological properties for cartilage tissue engineering, promoting chondrocyte proliferation. Our preliminary evidence contributes to the characterisation of HaeCs, highlighting the opportunity of using them for autologous cartilage implants in patients with haemophilia.
- Subjects :
- Cartilage, Articular drug effects
Cartilage, Articular pathology
Cell Proliferation drug effects
Cell Shape drug effects
Chondrocytes drug effects
Chondrocytes metabolism
Chondrocytes ultrastructure
Elastic Modulus drug effects
Female
Gene Expression Regulation drug effects
Hemophilia A genetics
Humans
Immunophenotyping
Male
Middle Aged
Polyvinyl Alcohol pharmacology
RNA, Messenger genetics
RNA, Messenger metabolism
Real-Time Polymerase Chain Reaction
Stress, Mechanical
Tissue Scaffolds
Transplantation, Autologous
Chondrocytes cytology
Chondrogenesis drug effects
Hemophilia A pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0878
- Volume :
- 366
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 27130570
- Full Text :
- https://doi.org/10.1007/s00441-016-2408-8