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Quantifying Aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2016 Aug 05; Vol. 165, pp. 120-126. Date of Electronic Publication: 2016 Apr 20. - Publication Year :
- 2016
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Abstract
- Rare earth doped upconversion nanoparticles convert near-infrared excitation light into visible emission light. Compared to organic fluorophores and semiconducting nanoparticles, upconversion nanoparticles (UCNPs) offer high photochemical stability, sharp emission bandwidths, and large anti-Stokes shifts. Along with the significant light penetration depth and the absence of autofluorescence in biological samples under infrared excitation, these UCNPs have attracted more and more attention on toxin detection and biological labelling. Herein, the fluorescence probe based on UCNPs was developed for quantifying Aflatoxin B1 (AFB1) in peanut oil. Based on a specific immunity format, the detection limit for AFB1 under optimal conditions was obtained as low as 0.2ng·ml(-1), and in the effective detection range 0.2 to 100ng·ml(-1), good relationship between fluorescence intensity and AFB1 concentration was achieved under the linear ratios up to 0.90. Moreover, to check the feasibility of these probes on AFB1 measurements in peanut oil, recovery tests have been carried out. A good accuracy rating (93.8%) was obtained in this study. Results showed that the nanoparticles can be successfully applied for sensing AFB1 in peanut oil.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Antibodies, Immobilized chemistry
Fluorescence
Food Analysis methods
Immunoassay methods
Limit of Detection
Magnetite Nanoparticles chemistry
Magnetite Nanoparticles ultrastructure
Metals, Rare Earth chemistry
Nanoparticles ultrastructure
Peanut Oil
Aflatoxin B1 analysis
Fluorescent Dyes chemistry
Nanoparticles chemistry
Plant Oils chemistry
Spectrometry, Fluorescence methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 165
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 27124091
- Full Text :
- https://doi.org/10.1016/j.saa.2016.04.040