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Genetically Engineered Ricin Suppresses Bactrocera dorsalis (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table.
- Source :
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Journal of economic entomology [J Econ Entomol] 2016 Apr 27; Vol. 109 (3), pp. 987-992. Date of Electronic Publication: 2016 Apr 27. - Publication Year :
- 2016
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Abstract
- The oriental fruit fly, Bactrocera dorsalis (Hendel), reduces the quantity and quality of many host fruits through the process of oviposition and larval feeding, and this insect has been considered a major insect pest in several Asian countries for decades. Using an earlier-developed, female-specific system that combines the toxicity of the ricin A chain (RTA) and the alternative RNA splicing property of doublesex ( Bddsx ), we show that transgenic male flies harboring the RTA-Bddsx transgene unevenly repress the pest population through inheritable effects. In age-stage, two-sex life-table analyses, high larval mortality and a delay in pupation were observed after introducing the transgene. The high male to female ratio in DsRed + flies demonstrates the lethal effect of ricin on females. The fitness of both the DsRed + - and DsRed - -transformed females was reduced as shown in the decrease of the net reproductive rate ( R0 ), intrinsic rate ( r ), and finite rate (λ) values compared with the wild-type populations. The integrity of the RTA-Bddsx transgene remained in more than 80% of DsRed + males after ten generations, supporting the stable inheritance of the transgene. All of the data from this study support the proposed RTA-Bddsx SIT approach, which provides a species-specific and environmentally friendly method of suppressing, rather than eradiating, B. dorsalis.<br /> (© The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Details
- Language :
- English
- ISSN :
- 1938-291X
- Volume :
- 109
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of economic entomology
- Publication Type :
- Academic Journal
- Accession number :
- 27122495
- Full Text :
- https://doi.org/10.1093/jee/tow091