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Immune responses to Mycoplasma bovis proteins formulated with different adjuvants.
- Source :
-
Canadian journal of microbiology [Can J Microbiol] 2016 Jun; Vol. 62 (6), pp. 492-504. Date of Electronic Publication: 2016 Feb 03. - Publication Year :
- 2016
-
Abstract
- Most vaccines for protection against Mycoplasma bovis disease are made of bacterins, and they offer varying degrees of protection. Our focus is on the development of a subunit-based protective vaccine, and to that end, we have identified 10 novel vaccine candidates. After formulation of these candidates with TriAdj, an experimental tri-component novel vaccine adjuvant developed at VIDO-InterVac, we measured humoral and cell-mediated immune responses in vaccinated animals. In addition, we compared the immune responses after formulation with TriAdj with the responses measured in animals vaccinated with a mix of a commercial adjuvant (Emulsigen™) and 2 of the components of the TriAdj, namely polyinosinic:polycytidylic acid (poly I:C) and the cationic innate defense regulator (IDR) peptide 1002 (VQRWLIVWRIRK). In this latter trial, we detected significant IgG1 humoral immune responses to 8 out of 10 M. bovis proteins, and IgG2 responses to 7 out of 10 proteins. Thus, we concluded that the commercial adjuvant formulated with poly I:C and the IDR peptide 1002 is the best formulation for the experimental vaccine.
- Subjects :
- Animals
Antimicrobial Cationic Peptides immunology
Arthritis immunology
Arthritis prevention & control
Arthritis veterinary
Bacterial Proteins immunology
Cattle
Cattle Diseases prevention & control
Chronic Disease
Female
Immunity, Cellular
Immunity, Humoral
Immunoglobulin G biosynthesis
Male
Mycoplasma Infections immunology
Mycoplasma Infections prevention & control
Pneumonia immunology
Pneumonia prevention & control
Pneumonia veterinary
Poly I-C immunology
Syndrome
Vaccines, Synthetic
Adjuvants, Immunologic
Antigens, Bacterial immunology
Bacterial Vaccines immunology
Cattle Diseases immunology
Mycoplasma Infections veterinary
Mycoplasma bovis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1480-3275
- Volume :
- 62
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Canadian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27105454
- Full Text :
- https://doi.org/10.1139/cjm-2015-0762