Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants.

Authors :: Mol JP
Pires SF
Chapeaurouge AD
Perales J
Santos RL
Andrade HM
Lage AP
Source :: PloS one [PLoS One] 2016 Apr 22; Vol. 11 (4), pp. e0154209. Date of Electronic Publication: 2016 Apr 22 (Print Publication: 2016).
Publication Year :: 2016
Abstract: Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.

Subjects :: Animals
Brucella abortus physiology
Brucellosis, Bovine microbiology
Cattle
Chorioallantoic Membrane microbiology
Culture Techniques
Electrophoresis, Gel, Two-Dimensional
Female
Host-Pathogen Interactions
Mass Spectrometry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Trophoblasts cytology
Trophoblasts metabolism
Trophoblasts microbiology
Brucellosis, Bovine metabolism
Chorioallantoic Membrane metabolism
Proteome metabolism
Proteomics methods

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