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Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2016 May 18; Vol. 8 (19), pp. 12048-55. Date of Electronic Publication: 2016 May 03. - Publication Year :
- 2016
-
Abstract
- A unique interaction between the cyanine dye and negatively charged quantum dot is used to construct a signal-on biaptameric quantum dot (QD) Förster resonance energy transfer (FRET) beacon for protein detection and distinct aptamer characterization. The beacon comprises a pair of aptamers, one intercalated with the cyanine dye (YOYO-3) and the other conjugated to a negatively charged, carboxyl-QD. When the target protein is present, structural folding and sandwich association of the two aptamers take place. As a consequence, YOYO-3 is displaced from the folded aptamer and transferred to the unblocked QD surface to yield a target concentration-dependent FRET signal. As a proof-of-principle, we demonstrate the detection of thrombin ranging from nanomolar to submicromolar concentrations and confirm the dye translocation using cylindrical illumination confocal spectroscopy (CICS). The proposed beacon provides a simple, rapid, signal-on FRET detection for protein as well as a potential platform for distinct aptamer screening.
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 8
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 27101438
- Full Text :
- https://doi.org/10.1021/acsami.6b02871