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Biflavonoid fraction from Garcinia kola seed ameliorates hormonal imbalance and testicular oxidative damage by anti-tuberculosis drugs in Wistar rats.
- Source :
-
Journal of basic and clinical physiology and pharmacology [J Basic Clin Physiol Pharmacol] 2016 Jun 01; Vol. 27 (4), pp. 393-401. - Publication Year :
- 2016
-
Abstract
- Background: Tuberculosis (TB) is a global health problem. The effects of anti-TB drugs on male reproductive system have not been properly evaluated. We investigated the effects of anti-TB drugs on testicular antioxidant indices, sperm characteristics and hormonal levels in rats, and the protective role of kolaviron (KV), a biflavonoid from Garcinia kola seed.<br />Methods: Twenty-eight male Wistar rats were assigned into four groups and orally treated with corn oil (control), anti-TB drugs [4-Tabs=isoniazid (5 mg/kg), rifampicin (10 mg/kg), pyrazinamide (15 mg/kg) and ethambutol (15 mg/kg) in combination], anti-TB drugs +KV and KV alone (200 mg/kg). Anti-TB drugs and KV were given three times per week for 8 weeks. In vitro, reducing power, inhibition of lipid peroxidation (LPO), diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging effects of KV were examined.<br />Results: KV at 10, 20, 50 and 100 μg/mL showed strong reducing potential and effectively scavenged DPPH and OH radicals in a concentration-dependent manner. Furthermore, KV significantly inhibited LPO in rats' liver homogenate. In vivo, administration of 4-Tabs caused a significant (p<0.05) decrease in body weight gain and weight of testis of rats. Body weight gain and weight of testis decreased by 45% and 36%, respectively, in the 4-Tabs-treated rats. Also, 4-Tabs increased testicular lipid peroxidation by 82%, with a concomitant decrease in antioxidant indices. Testicular reduced glutathione, superoxide dismutase and glutathione peroxidase decreased by 2.2-, 1.9- and 1.6-folds, respectively. Likewise, 4-Tabs markedly decreased sperm count, motility, luteinizing hormone and testosterone. Co-administration of KV with 4-Tabs normalized body weight, enhanced antioxidant system and improved sperm characteristics.<br />Conclusions: Kolaviron protects male reproductive system from oxidative damage by anti-tuberculosis drugs via the antioxidative mechanism.
- Subjects :
- Animals
Antioxidants metabolism
Biphenyl Compounds pharmacology
Flavonoids pharmacology
Glutathione metabolism
Glutathione Peroxidase metabolism
Lipid Peroxidation drug effects
Male
Picrates pharmacology
Rats
Rats, Wistar
Spermatozoa drug effects
Spermatozoa metabolism
Superoxide Dismutase metabolism
Testis drug effects
Testis metabolism
Antitubercular Agents pharmacology
Biflavonoids pharmacology
Garcinia kola chemistry
Hormones metabolism
Oxidative Stress drug effects
Plant Extracts pharmacology
Seeds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2191-0286
- Volume :
- 27
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of basic and clinical physiology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 27089414
- Full Text :
- https://doi.org/10.1515/jbcpp-2015-0063