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Humibacter soli sp. nov., isolated from soil.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2016 Jul; Vol. 66 (7), pp. 2509-2514. Date of Electronic Publication: 2016 Apr 15. - Publication Year :
- 2016
-
Abstract
- A novel actinobacterial strain, designated R1-20 <superscript>T</superscript> , was isolated during a study of the bacterial diversity of the soil at a white heron nesting site. The isolate was non-motile, Gram-stain-positive and short rod-shaped. Colonies were dull white and convex with entire margin during the early stages of growth, and gradually became yellow. 16S rRNA gene sequence analysis indicated that the isolate belongs to the genus Humibacter of the family Microbacteriaceae, as sequence similarity with its nearest neighbours was 97.16 % with Humibacter antriD7-27 <superscript>T</superscript> and 96.44 % with Humibacter albusDSM 18994 <superscript>T</superscript> . However, the combination of cultural and physiological as well as chemotaxonomic properties clearly distinguished strain R1-20 <superscript>T</superscript> from other Humibacter species. The DNA G+C content of strain R1-20 <superscript>T</superscript> was 65.5 mol%, and the major respiratory isoprenoid quinone was menaquinone MK-11. The acyl group of the peptidoglycan was of acetyl type, and the diagnostic diamino acid was 2,4-diaminobutyric acid. Glutamic acid, alanine and glycine were also present in the cell wall. The major fatty acids of strain R1-20 <superscript>T</superscript> were anteiso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. On the basis of phylogenetic, phenotypic and chemotaxonomic analysis, strain R1-20 <superscript>T</superscript> merits recognition as a representative of a novel species of the genus Humibacter, for which the name Humibacter soli sp. nov. is proposed. The type strain is R1-20 <superscript>T</superscript> (=KCTC 39614 <superscript>T</superscript> =JCM 31015 <superscript>T</superscript> ).
- Subjects :
- Actinomycetales genetics
Actinomycetales isolation & purification
Aminobutyrates chemistry
Bacterial Typing Techniques
Base Composition
Cell Wall chemistry
DNA, Bacterial genetics
Fatty Acids chemistry
Peptidoglycan chemistry
RNA, Ribosomal, 16S genetics
Republic of Korea
Sequence Analysis, DNA
Vitamin K 2 chemistry
Actinomycetales classification
Phylogeny
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 66
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27081846
- Full Text :
- https://doi.org/10.1099/ijsem.0.001081