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Phylogenetic Diversity of Ammopiptanthus Rhizobia and Distribution of Rhizobia Associated with Ammopiptanthus mongolicus in Diverse Regions of Northwest China.
- Source :
-
Microbial ecology [Microb Ecol] 2016 Jul; Vol. 72 (1), pp. 231-239. Date of Electronic Publication: 2016 Apr 14. - Publication Year :
- 2016
-
Abstract
- Aiming to investigate the diversity and distribution of rhizobia associated with Ammopiptanthus, an endangered evergreen legume widely distributed in deserts, we characterized a total of 219 nodule isolates from nine sampling sites in Northwest China with different soil characteristics based upon restriction fragment length polymorphism (RFLP) analysis of 16S ribosomal RNA (rRNA) and symbiotic genes (nodC and nifH). Ten isolates representing different 16S rRNA-RFLP types were selected for further sequence analyses of 16S rRNA and four housekeeping genes. As results, nine genospecies belonging to the genera Ensifer, Neorhizobium, Agrobacterium, Pararhizobium, and Rhizobium could be defined among the isolates. The nodC and nifH phylogenies of 14 isolates representing different symbiotic-RFLP types revealed five lineages linked to Ensifer fredii, Ensifer meliloti, Rhizobium leguminosarum, Mesorhizobium amorphae, and Rhizobium gallicum, which demonstrated the various origins and lateral transfers of symbiotic genes between different genera and species. The rhizobial diversities of Ammopiptanthus mongolicus varied among regions, and the community compositions of rhizobia associated with A. mongolicus were significantly different in wild and cultured fields. Constrained correspondence analysis showed that the distribution of A. mongolicus rhizobia could be explained by available potassium content and that the assembly of symbiotic types was mainly affected by available phosphorus content and carbon-nitrogen ratio.
- Subjects :
- Biodiversity
China
DNA, Bacterial genetics
Evolution, Molecular
Genes, Bacterial
Genetic Variation
Mesorhizobium genetics
Mesorhizobium isolation & purification
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S genetics
Rhizobium genetics
Rhizobium isolation & purification
Sequence Analysis, DNA
Symbiosis
Fabaceae microbiology
Phylogeny
Rhizobium classification
Subjects
Details
- Language :
- English
- ISSN :
- 1432-184X
- Volume :
- 72
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial ecology
- Publication Type :
- Academic Journal
- Accession number :
- 27079453
- Full Text :
- https://doi.org/10.1007/s00248-016-0759-z