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Hepatic stellate cell promoted hepatoma cell invasion via the HGF/c-Met signaling pathway regulated by p53.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2016; Vol. 15 (7), pp. 886-94. - Publication Year :
- 2016
-
Abstract
- The biological behaviors of hepatocellular carcinoma (HCC) are complex mainly due to heterogeneity of progressive genetic and epigenetic mutations as well as tumor environment. Hepatocyte growth factor (HGF)/c-Met signaling pathway is regarded to be a prototypical example for stromal-epithelial interactions during developmental morphogenesis, wound healing, organ regeneration and cancer progression. And p53 plays as an important regulator of Met-dependent cell motility and invasion. Present study showed that 2 HCC cell lines, Hep3B and HepG2, displayed different invasive capacity when treated with HGF which was secreted by hepatic stellate cells (HSCs). We found that HGF promoted Hep3B cells invasion and migration as well as epithelial-mesenchymal transition (EMT) occurrence because Hep3B was p53 deficient, which leaded to the c-Met over-expression. Then we found that HGF/c-Met promoted Hep3B cells invasion and migration by upregulating Snail expression. In conclusion, HGF/c-Met signaling is enhanced by loss of p53 expression, resulting in increased ability of invasion and migration by upregulating the expression of Snail.
- Subjects :
- Carcinoma, Hepatocellular enzymology
Carcinoma, Hepatocellular genetics
Carcinoma, Hepatocellular pathology
Cell Line
Cell Line, Tumor
Cell Movement
Hep G2 Cells
Hepatic Stellate Cells metabolism
Hepatocyte Growth Factor metabolism
Humans
Liver Neoplasms enzymology
Liver Neoplasms genetics
Liver Neoplasms pathology
Neoplasm Invasiveness
Proto-Oncogene Proteins c-met physiology
Signal Transduction
Snail Family Transcription Factors metabolism
Carcinoma, Hepatocellular metabolism
Hepatic Stellate Cells physiology
Hepatocyte Growth Factor physiology
Liver Neoplasms metabolism
Proto-Oncogene Proteins c-met metabolism
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 15
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 27077227
- Full Text :
- https://doi.org/10.1080/15384101.2016.1152428