Fibronectin-modified surfaces for evaluating the influence of cell adhesion on sensitivity of leukemic cells to siRNA nanoparticles.

Authors :: Valencia-Serna J
Chevallier P
Kc RB
Laroche G
Uludağ H
Source :: Nanomedicine (London, England) [Nanomedicine (Lond)] 2016 May; Vol. 11 (9), pp. 1123-38. Date of Electronic Publication: 2016 Apr 13.
Publication Year :: 2016
Abstract: Aim: This study aimed to create fibronectin (FN)-grafted polymeric surfaces to investigate the influence of leukemic cell adhesion on siRNA treatment.<br />Materials & Methods: FN was grafted on plasma-treated PTFE surfaces using chemical crosslinkers. Adhesion and growth of chronic myeloid leukemia K562 cells on modified surfaces were investigated. The silencing effect of siRNA/lipid-polymers nanoparticles on cells grown on FN-grafted surfaces was evaluated.<br />Results: Crosslinker-mediated immobilization showed significant FN grafting on surfaces, which provided K562 cell adhesion and growth advantage. siRNA nanoparticle silencing was similarly effective on FN-adhered and suspension-growing K562 cells.<br />Conclusion: This study provided initial data to develop a cell-adhesive system to investigate therapeutic effects on leukemic cells. The response of chronic myeloid leukemia cells to siRNA nanoparticles was independent on cell attachment.

Subjects :: Biocompatible Materials administration & dosage
Biocompatible Materials chemistry
Cell Adhesion genetics
Fibronectins administration & dosage
Fibronectins chemistry
Genetic Therapy
Humans
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology
Nanoparticles administration & dosage
Nanoparticles chemistry
RNA, Small Interfering administration & dosage
Surface Properties
Fibronectins genetics
Gene Transfer Techniques
Leukemia, Myelogenous, Chronic, BCR-ABL Positive therapy
RNA, Small Interfering genetics

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