Back to Search Start Over

Batch and fed-batch bioreactor studies for the enhanced production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428.

Authors :
Kumar S
Prabhu AA
Dasu VV
Pakshirajan K
Source :
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2017 Jan 02; Vol. 47 (1), pp. 74-80. Date of Electronic Publication: 2016 Apr 12.
Publication Year :
2017

Abstract

The effect of dissolved oxygen (DO) level and pH (controlled/uncontrolled) was first studied to enhance the production of novel glutaminase-free L-asparaginase by Pectobacterium carotovorum MTCC 1428 in a batch bioreactor. The optimum level of DO was found to be 20%. The production of L-asparaginase was found to be maximum when pH of the medium was maintained at 8.5 after 12 h of fermentation. Under these conditions, P. carotovorum produced 17.97 U/mL of L-asparaginase corresponding to the productivity of 1497.50 U/L/h. The production of L-asparaginase was studied in fed-batch bioreactor by feeding L-asparagine (essential substrate for production) and/or glucose (carbon source for growth) at the end of the reaction period of 12 h. The initial medium containing both L-asparagine and glucose in the batch mode and L-asparagine in the feeding stream was found to be the best combination for enhanced production of glutaminase-free L-asparaginase. Under this condition, the L-asparaginase production was increased to 38.8 U/mL, which corresponded to a productivity of 1615.8 U/L/h. The production and productivity were increased by 115.8% and 7.9%, respectively, both of which are higher than those obtained in the batch bioreactor experiments.

Details

Language :
English
ISSN :
1532-2297
Volume :
47
Issue :
1
Database :
MEDLINE
Journal :
Preparative biochemistry & biotechnology
Publication Type :
Academic Journal
Accession number :
27070115
Full Text :
https://doi.org/10.1080/10826068.2016.1168841